This interactive software, written by Daniel Segre', computes metabolic fluxes for E. coli wild type and user-selected mutants, using FBA and MOMA. For more info on these methods, see here. This online demo uses the E. coli stoichiometric reconstruction published in Edwards and Palsson, PNAS 2000. For more recent E. coli stoichiometric reconstructions, see here)

Optimization options:

Mutate Isozymes YES NO
Constrain all fluxes relative to enzyme(s) YES NO
Display all fluxes in output YES NO

Nutrient uptake upper bounds
Glucose Oxygen Nitrogen

Genes to mutate:
* Select box -> delete
* Click on gene name -> link to EcoCyc
glk1 pgi1 pgi2 pgi3 galm agp
pfka pfkb1 fbp fba tpia gapa
gapc pgk gpma gpmb eno ppsa
pyka pykf acee glgc glga glgp
malp zwf pgl gnd rpia rpib
rpe tkta1 tktb1 tkta2 tktb2 talb
edd eda glta acna acnb icda
suca succ sdha1 frda fuma fumb
fumc mdh dld1 ldha adhe2 pfla
pflc fdhf1 pta acka purt1 acs
pcka ppc maeb sfca acea aceb
glcb ppa ndh nuoa fdng fdoh
fdhf2 poxb dld2 glpd glpa cyoa
cyda sdha2 trxb pnt1a pnt2a hyaa